Salicylic acid-mediated diacylglycerol/triacylglycerol conversion affects the freezing tolerance of Arabidopsis

Diacylglycerol (DAG) is likely converted to triacylglycerol (TAG) by the enzyme diacylglycerol acyltransferase (DGAT), and this conversion important in freezing tolerance of Arabidopsis. The phytohormone salicylic acid (SA) DAG/TAG ratio are known play roles chilling Arabidopsis while interaction or connection was not clear. In our study, we analysed phenotype, proline sugar accumulation, content, lipid profiling dgat1 mutants during stress. We found that dgat1-1 exhibited higher sensitivity long time cold stress showed lower accumulation under acclimation conditions. freezing-sensitive phenotype can be ameliorated mutations key SA signalling components SAG101, EDS1, PAD4 through phenotyping analysis double mutants. Dgat1 accumulated more SA, ABA (abscisic acid), JA-Ile (jasmonate isoleucine), OPDA (12-oxyphytodienoic acid) after recovery. addition, content SA-deficient mutant sid2 than wild type, SA-excessive siz1 opposite trend. summary, could negatively mediate regulating DAG TAG, which influences integrity membrane it provide a novel research direction.